Expression and Characterization of Geotrichum candidum Lipase I Gene. Comparison of Specificity Profile with Lipase II
نویسندگان
چکیده
منابع مشابه
Elucidation of the effects of inoculum size and age on lipase production by Geotrichum candidum
Lipases are extremely versatile enzymes with large industrial applications and they have been the subject of investigation over the last few years. A great variety of microorganisms can produce this enzyme, such as pluricellular fungi, but their growth need to be optimized due to the variations amongst experiments, which influence the results and reproducibility of the process. The aim of this ...
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Mycelium-bound lipase (MBL) was prepared using a strain of Geotrichum candidum isolated from local soil. At the time of maximum lipase activity (54 h), the mycelia to which the lipase was bound were harvested by filtration and centrifugation. Dry MBL was prepared by lyophilizing the mycelia obtained. The yield of MBL was 3.66 g/l with a protein content of 44.11 mg/g. The lipase activity and spe...
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The three-dimensional structure of lipase II of Geotrichum candidum strain ATCC34614 (GCL II) has provided insights with respect to the nature of the catalytic machinery of lipases. To support these structural observations, we have carried out an analysis of GCL II by mutagenesis. The gene encoding lipase II of Geotrichum candidum strain ATCC34614 (GCL II) was amplified using the polymerase cha...
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15 صفحه اولDisseminated Geotrichum candidum infection.
The pathogenicity of the fungus Geotrichum candidum in humans has not been clearly defined. A patient with acute leukemia who developed a fatal disseminated G. candidum infection while neutropenic is described. At autopsy examination, this patient was misdiagnosed on the basis of histopathology as having disseminated candidiasis until G. candidum was isolated from postmortem culture specimens. ...
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ژورنال
عنوان ژورنال: European Journal of Biochemistry
سال: 1995
ISSN: 0014-2956,1432-1033
DOI: 10.1111/j.1432-1033.1995.0863m.x